Flow Cytometry-Based Tools to Enrich Your Preclinical Immuno-Oncology Research

Overview: Flow cytometry provides a highly adaptable and quantitative method for investigating both the cellular distribution and intracellular changes that occur in tumors and peripheral lymphoid organs in response to therapy. As clinical successes with cancer immunotherapy accumulate, preclinical immuno-oncology research depends on greater mechanistic understanding of novel therapeutic strategies. […]

Multiplex IHC for Patterns of Protein Expression, Co-expression, and Spatial Relationships

Multiplex immunohistochemistry (IHC) is an assay that utilizes the basic concept of single antigen staining to detect multiple markers at one time. It allows for the labeling capabilities of peroxidase, alkaline phosphatase, fluorescence, and other conjugated primary or secondary antibodies and can be achieved using several common methods including indirect or direct staining protocols. Researchers […]

Phospho-Protein Detection in Solid Tumors Using Phospho-Flow Cytometry

About the Author: Dr. David Draper is an immunologist and senior member of the Flow Cytometry Team at MI Bioresearch since 2015. Dr. Draper holds a Ph.D. in Microbiology from North Carolina State University. His post-doctoral work at Duke University and the National Institutes of Health focused on uncovering the […]

An Introduction to Immunohistochemistry

Immunohistochemistry (IHC) is an assay that utilizes the biological collaboration of Histology and Immunology. The biochemical interactions between an antibody and antigen permit visual distribution and localization of antibody–antigen interactions. This allows morphology, antigen intensity, and spatial relationships to be determined. MI Bioresearch offers high-quality IHC assays onsite to give you a time-efficient read-out on […]

Use of Luminex® Technology to Quantify Biomarkers and Provide Agent Efficacy

In recent years many researchers have focused on the analysis of circulating soluble cancer biomarkers as an indicator of the host immune response to oncogenesis.1,2,3 Multiple biomarkers can be quantified in order to fully characterize changes in homeostasis brought on by tumor growth and metastasis. The ability to multiplex tumor-type specific antigens and general immune […]

Flow Cytometry Based Functional Assays Essential for Characterizing Biological Significance

NK Cell Stimulation: Splenocytes or dissociated tumors are cultured with IL-12 and IL-18, treated to prevent excretion of produced cytokines. Expression of markers including IFN-γ are quantified to identify activated NK cells within the total NK cell population. B-cell Stimulation: Splenocytes or dissociated tumors are cultured with IgM and expression […]

Get Glowing Results From Your Cell Lines – With Luciferase

Since different cell types use different promoters to express genes, a variety of promoters including EF1 alpha, CMV, UbC, and MSCV can be used. Also, for high-efficiency gene delivery, the pseudotype of the virus can be modified to VSV-G or MLV. If you are interested in luc-enabling your cell lines, contact […]