5TGM1-luc – A Syngeneic Murine Model for Multiple Myeloma

The 5TGM1 multiple myeloma model arose spontaneously in aging C57BL/KaLwRij mice, and was propagated via serial passage in syngeneic mice prior to establishment of a cell line. MI Bioresearch’s 5TGM1 line has been luciferase-enabled to permit monitoring of growth in orthotopic sites such as the bone marrow by bioluminescent imaging […]

Flow Cytometry Based Functional Assays Essential for Characterizing Biological Significance

NK Cell Stimulation: Splenocytes or dissociated tumors are cultured with IL-12 and IL-18, treated to prevent excretion of produced cytokines. Expression of markers including IFN-γ are quantified to identify activated NK cells within the total NK cell population. B-cell Stimulation: Splenocytes or dissociated tumors are cultured with IgM and expression […]

In-Depth Myeloid Cell Characterization in the Murine Syngeneic CT.26 Colon Carcinoma Model by 10-Color Flow Cytometry

The efficacy of immune-modulating anti-cancer therapeutic antibodies that have been FDA-approved in recent years, such as anti-CTLA-4 and anti-PD-1, has driven growing interest in methods that provide a mechanistic understanding of drug function. Development of new mono- and combination therapies with immune-modulatory effects requires more powerful immunophenotyping techniques capable of in depth cell characterization. During […]

Evaluation of the Immune Response Following Treatment with Anti-CTLA-4 Antibody, Radiation Therapy, or the Combination in Murine Model of Breast Cancer

Breast cancers are considered poorly immunogenic tumors, however, several approaches utilizing immunotherapies are being undertaken in the clinic to evaluate their potential for improving outcomes. Radiation therapy (RT) is a highly utilized clinical treatment modality in breast cancer. Radiation is known to modify the tumor microenvironment, induce cytokines and chemokines, and has been shown to […]

CT26: Murine Colon Carcinoma

CT26 and Flow Cytometry In addition to evaluating efficacy of anti-cancer drug combinations this model has proven useful in the assessment of phenotypic changes in the immune system. To this end we have evaluated CT26 tumors for a number of fundamental tumor infiltrating leukocytes with our Attune NxT® flow cytometer. […]

Get Glowing Results From Your Cell Lines – With Luciferase

Since different cell types use different promoters to express genes, a variety of promoters including EF1 alpha, CMV, UbC, and MSCV can be used. Also, for high-efficiency gene delivery, the pseudotype of the virus can be modified to VSV-G or MLV. If you are interested in luc-enabling your cell lines, contact […]