MI Bioresearch offers in-house histology and immunohistochemistry (IHC), further expanding our extensive list of services to enhance your study. We are committed to making your outsourcing as easy and streamlined as possible. Optimal preservation and structural integrity of your samples is ensured by simply moving samples down the hallway from the vivarium to the histology lab in our state-of-the-art facility. Quality imaging for analysis is achieved using the Aperio VERSA Brightfield Fluorescence Digital Pathology Scanner. MI offers a full suite of histology and IHC services, including custom work, and will be adding markers routinely.

Our high-quality IHC assays give you a time-efficient read-out on quantitative and qualitative data and can be produced expeditiously that reveal the following:

  • Efficacy of your compound in target tissues
  • Location of biomarkers in tissues
  • Incidence of tumor metastasis
  • Occurrence of tissue transformation into tumors
  • Origin of tumor tissue
  • Prognosis of the animals in the study

Featured IHC Articles:

Services:

  • Normal Tissue
  • Tumor Tissue
  • Xenograft
  • New Marker Validation
  • Immunofluorescence
  • Immunocytochemistry
  • Multiplex – Custom markers with up to four-color immunofluorescence

Validated IHC Markers:

AE1/AE3+5D3

AE1/AE3+5D3 cytoplasmic positive mouse skin. Cytokeratin's are filamentous proteins detectable in epithelial cells. They can be utilized in tumor differentiation, cell typing, and micrometastasis.

AE1/AE3+5D3 cytoplasmic positive mouse skin. Cytokeratin’s are  filamentous proteins detectable in epithelial cells. They can be utilized in tumor differentiation, cell typing, and micrometastasis.

CD3

Image of CD3 membrane staining in spleen.
CD3 membrane staining in spleen
Image of CD3 staining in 4T1.
CD3 staining in 4T1
Image of CD3 staining in A20.
CD3 staining in A20
Image of CD3 staining in CT26.
CD3 staining in CT26

Mouse CD3 membrane staining in spleen. CD3 is used to identify T cells in normal tissue, as well as T cell neoplasms.

CD4

Image of CD4 staining in A20.
CD4 staining in subcutaneous A20
Image of CD4 immunofluorescent staining in A20.
CD4 immunofluorescent staining in A20
Image of CD4 staining in CT26.
CD4 staining in CT26
Image of CD4 staining in Pan02.
CD4 staining in Pan02

Mouse CD4 membrane staining in mouse tumor tissue. CD4 is used to identify T helper cells, and plays a role in T cell activation.

CD8

CD8

Mouse CD8 membrane staining in subcutaneous CT26 mouse tumor tissue. CD8 is used to identify cytotoxic T cells.

CD19

CD19_Fluorescence

Human CD19 membrane staining in human tonsil. CD19 is a biomarker for B cells, and plays a role in B cell development.

CD20

CD20 Staining Image

Human CD20 staining in human tonsil. CD20 is a B cell marker whose function is to enable optimal B-cell immune response.

CD22

CD22 Staining Image

Human CD22 staining in human tonsil. CD22 is found on the surface of mature B cells that functions as an inhibitory receptor for B cell receptor signaling. It is an active target for B cell malignancies and autoimmune disease.

CD38

CD38 staining in 4T1
CD38 staining in 4T1
CD38 staining in A20
CD38 staining in A20
CD38 staining in CT26
CD38 staining in CT26
CD38 staining in ID8
CD38 staining in ID8

Mouse CD38 staining in multiple tissues, as indicated. CD38 is found on the surface of many immune cells (CD4+ and CD8+ T cells, B cells, and natural killer cells). It has functions in cell adhesion, signal transduction and calcium signaling and is used as a prognostic marker in leukemia patients with CLL.

CD45

Mouse CD45 membrane staining in A20 tumor tissue. CD45 plays a role in the regulation of T- and B-cell antigen receptor signaling.

Mouse CD45 membrane staining in A20 tumor tissue. CD45 plays a role in the regulation of T- and B-cell antigen receptor signaling.

CD163

CD163 staining in CT26
CD163 staining in CT26
CD163 staining in EMT6
CD163 staining in EMT6
CD163 staining in HEPA-1-6
CD163 staining in HEPA-1-6
CD163 staining in ID8
CD163 staining in ID8

Mouse CD163 staining in multiple tissues, as indicated. CD163 is expressed in macrophages and monocytes. Expression may be indicative of inflammation.

FoxP3

FoxP3

Mouse FOXP3 Nuclear staining in Spleen. FoxP3 is involved in the development and inhibitory function of regulatory T cells.

HER2

HER2 with arrows (BT474 tumor tissue)
Her2 positive cytoplasm in BT474 tumor tissue.

 

HER2_Fluorescence (SK-OV-3 Cells)
HER2 Immunofluorescence of SK-OV-3 Cells

Her2 is a human EGF receptor involved in kinase mediated activation of signaling pathways. It is used as a predictive factor and determinant in therapy in cancer treatment.

Ki67

Ki67 positive tonsil tissue, with localization dominant in the nucleus. The Ki67 protein Is associated cellular proliferation, and therefore can be used as a prognostic tool in cancer.

Human Ki-67 nuclear staining in human tonsil. The Ki-67 protein is associated with cellular proliferation, and can be used as a prognostic tool in cancer.

PR

PR with arrows

Human PR positive nuclei in BT474 tumor tissue. Progesterone receptor is involved in activating signaling pathway for hormone stimulation. It can be utilized in determining appropriate drug  therapy in cancer treatment.