A Deeper Dive into the Tumor-Infiltrating T Cell Immunophenotype

A Deeper Dive into the Tumor-Infiltrating T Cell Immunophenotype – Introducing the New MI-Expanded CompT™ Multi-Color Flow Cytometry Panel Flow cytometry using large antibody panels has the advantage of examining a greater number of cell subsets. This capability is particularly important when a comprehensive data set is required, but limited […]

Comprehensive Dendritic Cell Analysis by Flow Cytometry Using the New MI-CompDC™ Panel

Table 1: MI-CompDC™ Panel Antibodies and Description of Their Utility Antibody/Dye Description CD45 Pan immune cell marker F4/80, Ly-6G, CD19 Exclusion gate for macrophages, granulocytes, and B cells CD11c Dendritic cell marker CD24 Dendritic cell marker CD8 DC1 marker MHC Class II Dendritic cell marker/maturation marker CD103 DC1 marker CD11b […]

Multiplex IHC for Patterns of Protein Expression, Co-expression, and Spatial Relationships

Multiplex immunohistochemistry (IHC) is an assay that utilizes the basic concept of single antigen staining to detect multiple markers at one time. It allows for the labeling capabilities of peroxidase, alkaline phosphatase, fluorescence, and other conjugated primary or secondary antibodies and can be achieved using several common methods including indirect or direct staining protocols. Researchers […]

Phospho-Protein Detection in Solid Tumors Using Phospho-Flow Cytometry

About the Author: Dr. David Draper is an immunologist and senior member of the Flow Cytometry Team at MI Bioresearch since 2015. Dr. Draper holds a Ph.D. in Microbiology from North Carolina State University. His post-doctoral work at Duke University and the National Institutes of Health focused on uncovering the […]