High-Throughput Analysis of MAPK and JAK-STAT Signaling in CT26 Tumors Using a Combination of Immunophenotyping and Phospho-Flow Cytometry

“High-Throughput Analysis of MAPK and JAK-STAT Signaling in CT26 Tumors Using a Combination of Immunophenotyping and Phospho-Flow Cytometry” Introduction and Background The development of robust platforms that enable phospho-protein-based analysis of cell signaling in the tumor microenvironment is pivotal for small molecule drug development. Currently lacking are reliable applications that […]

Evaluation of Immunomodulatory Agents in Classically Immunologically “Cold” Cancers Using Syngeneic Mouse Models of Breast and Ovarian Cancer

“Evaluation of Immunomodulatory Agents in Classically Immunologically “Cold” Cancers Using Syngeneic Mouse Models of Breast and Ovarian Cancer“ Introduction and Background T cell checkpoint inhibitors have demonstrated significant clinical benefit in immunologically “hot” cancer types like melanoma, kidney, bladder and lung cancers. “Hot” tumors are characterized by a significant CD8+ […]

Radiation Enhances Anti-Tumor Activity of Checkpoint Blockade in Syngeneic Tumor Models

“Radiation Enhances Anti-Tumor Activity of Checkpoint Blockade in Syngeneic Tumor Models” Introduction and Background More than 50% of cancer patients receive some sort of radiation therapy during the course of their illness. While radiation treatment is a mainstay in clinical oncology, there is limited preclinical data in syngeneic models. The […]

A Comparative Evaluation of Immunotherapy Responses in Murine Colon Carcinoma

“A Comparative Evaluation of Immunotherapy Responses in Murine Colon Carcinoma” Introduction and Background With the drive in oncology to develop molecules targeting the immune system, there is a need for reliable and well-characterized preclinical models. The focus of immuno-oncology drug development has shifted to combination strategies with approved immunotherapies, immunometabolism […]

Sensitivity of Syngeneic Tumor Models to Focal Radiation

“Sensitivity of Syngeneic Tumor Models to Focal Radiation” Introduction and Background Use of image-guided focal irradiation is a mainstay of human cancer treatment. Image guidance allows for highly conformal treatment plans that minimize normal tissue toxicity and systemic response. With the advent of image-guided small animal irradiators such as the […]

Preclinical Models of Multiple Myeloma

“Preclinical Models of Multiple Myeloma” Introduction and Background Multiple myeloma is a clonal B cell malignancy characterized by the accumulation of terminally differentiated, antibody-producing plasma cells in the bone marrow that is invasive and drug resistant. Patients are generally asymptomatic until very late state disease. There are few preclinical models […]

Characterization of Proliferation in Multiple Lymphocyte Subsets in the CT26 Murine Colon Carcinoma Model by Multi-color Flow Cytometry

“Characterization of Proliferation in Multiple Lymphocyte Subsets in the CT26 Murine Colon Carcinoma Model by Multi-color Flow Cytometry” Introduction and Background: The efficacy of immune-modulating anti-cancer therapeutic antibodies that have been FDA-approved in recent years, such as anti-CTLA-4, anti-PD-1, and anti-PD-L1, has altered the paradigm of cancer treatment. Subsequently, the […]