MM.1S: A Model for Multiple Myeloma

Mean Total Tumor Burden BLI Signal In SCID beige mice, the median tumor volume doubling time is ~2 days (Figure 3), and median time to morbidity/mortality endpoint is 35-45 days. To further optimize the MM.1S-pMMP-LucNeo model, we characterized growth in the NSG mouse strain which shows more aggressive disease onset […]

Preclinical Success to Clinical Failure: Do We Have a Model Problem or an Endpoint Problem?

As the AACR (American Association for Cancer Research) Annual Meeting is fast approaching, many industry and academic scientists are busy preparing talks and posters for what they hope will be the next new wave in cancer therapy or the next “new and improved” preclinical model. However, while these may be long shots, it’s the scientific […]

Colo-205 as a Translational Imaging Model for Colorectal Cancer

As mentioned in our HT-29 Model Spotlight last October, colorectal cancer (CRC) is the second leading cause of cancer-related deaths in the United States for men and women combined. The death rate from CRC has been declining over the past several decades and there are now more than one million CRC survivors in the United […]

Use of Luminex® Technology to Quantify Biomarkers and Provide Agent Efficacy

In recent years many researchers have focused on the analysis of circulating soluble cancer biomarkers as an indicator of the host immune response to oncogenesis.1,2,3 Multiple biomarkers can be quantified in order to fully characterize changes in homeostasis brought on by tumor growth and metastasis. The ability to multiplex tumor-type specific antigens and general immune […]

PC-3M-Luc-C6 – A Model for Prostate Carcinoma

Orthotopic Modeling In an effort to address this unmet need, MI Bioresearch has developed an orthotopic and a metastatic prostate model, PC-3M-Luc-C6. This cell line has been transfected with luciferase, which allows for disease and response monitoring through bioluminescence imaging (BLI). The orthotopic model is implanted directly into the prostate […]

4T1-luc2: An Orthotopic Mammary Cancer Model to Support Novel Immuno-Oncology Drug Discovery

Breast cancer is the second most deadly malignancy after lung cancer in woman in the United States, with an estimated 246,000 new cases and 40,450 deaths expected in 2016. Many treatment options for breast cancer exist including surgery, radiotherapy, anti-estrogen therapy, targeted therapies (e.g., trastuzumab), and chemotherapy. Despite these therapeutic advances, metastatic disease remains a […]

Measuring Extracellular pH Within Tumors Using CEST MRI

pH is one of the key microenvironmental factors in the development of tumors. Tumor cells are often viewed as high lactate and H+ producers.1 Extracellular acidosis represents a threat to cell survival by modifying the intracellular pH (pHi), wherein a 0.1 pHi variation can disrupt multiple biological functions.2 Measurements of tumor extracellular pH (pHe) may […]

Immune Profile of the Neuro-2a Mouse Neuroblastoma Model by Flow Cytometry

Neuroblastoma is an extracranial tumor that arises from neural crest-derived progenitor cells. It is the most prevalent solid tumor in childhood and the most common tumor in infants less than one year of age. With up to 800 new cases diagnosed each year, in spite of aggressive multi-modal therapeutic approaches, the 5-year survival rate in […]

HT-29 as a Preclinical Model for Colorectal Cancer

In this model we have also evaluated the combination of radiation and gemcitabine (Figure 5) and illustrate the added benefit of the combination over either single agent treatment. Fig. 5: Subcutaneous Growth of HT-29 Following Combination Treatment with Localized Radiation and Gemcitabine Contact MI Bioresearch to discuss your next preclinical […]

Longitudinal Evaluation of Orthotopic Lung Tumors With Imaging

Clinical imaging technologies have been vital for detecting and following disease progression/regression for many years, as examples, Computed Tomography (CT) can measure tumor volume over time and Positron Emission Tomography (PET) can measure metabolic activity within the tumors. At MI Bioresearch we have been utilizing preclinical imaging technologies for over […]

786-O (pMMP-LucNeo) – A Model for Renal Cell Carcinoma

In an effort to address this unmet need, MI Bioresearch has developed an orthotopic RCC model, 786-O (pMMP-LucNeo) through implantation into the sub-renal capsule. This cell line has been transfected with luciferase, which allows for disease and response monitoring through bioluminescence imaging (BLI). In 786-O tumor bearing mice, we evaluated […]

Leveraging the Clinical Power of MRI in Preclinical Liver Tumor Imaging

The liver is an ideal organ for magnetic resonance imaging (MRI) due to its size, plus the inherent contrast it enables compared to other tissues and lesions; this results from the high iron content which acts as a natural MRI contrast agent. For such reasons, liver tumor MRI is a major area of clinical and […]

5TGM1-luc – A Syngeneic Murine Model for Multiple Myeloma

The 5TGM1 multiple myeloma model arose spontaneously in aging C57BL/KaLwRij mice, and was propagated via serial passage in syngeneic mice prior to establishment of a cell line. MI Bioresearch’s 5TGM1 line has been luciferase-enabled to permit monitoring of growth in orthotopic sites such as the bone marrow by bioluminescent imaging […]

Approaches for Preclinical Evaluation of Ovarian Cancer

As many patients with ovarian cancer develop peritoneal ascites, one standard preclinical method is to deliver these cell lines intraperitoneally (IP) to mimic this disease state. With our luciferase expressing lines we are able to track and monitor disease establishment and progression with BLI and report out quantitative measurements of […]

A549 – A Model for Non-Small Cell Lung Cancer

At MI Bioresearch we have been using A549 as a model of NSCLC for more than 10 years. We have run numerous studies in the subcutaneous setting with a number of standard agents. Above are some examples of response to paclitaxel (Figure 1) and docetaxel, bevacizumab and the combination (Figure […]

In-Depth Myeloid Cell Characterization in the Murine Syngeneic CT.26 Colon Carcinoma Model by 10-Color Flow Cytometry

The efficacy of immune-modulating anti-cancer therapeutic antibodies that have been FDA-approved in recent years, such as anti-CTLA-4 and anti-PD-1, has driven growing interest in methods that provide a mechanistic understanding of drug function. Development of new mono- and combination therapies with immune-modulatory effects requires more powerful immunophenotyping techniques capable of in depth cell characterization. During […]

MDA-MB-231-luc-D3H2LN: A Valuable Model for Triple-Negative Breast Cancer Research

Fig 3:  MDA-MB-231-luc-D3H2LN Mammary Fat Pad Orthotopic Implant – Lymph Nodes MI Bioresearch has also validated MDA-MB-231-luc-D3H2LN as a metastatic bone model following an intracardiac injection. The MDA-MB-231-luc-D3H2LN cells naturally hone to the bone. Utilizing bioluminescence imaging will ensure that the injections are successful and that treatment groups can be […]

Comparison of Bioluminescence and MR Imaging to Evaluate Orthotopic Glioma Models

Glioblastoma multiforme (GBM) (also called glioblastoma) is a fast-growing glioma that develops from star-shaped glial cells (astrocytes and oligodendrocytes) that support the health of the nerve cells within the brain. These tumors are usually highly malignant because the cells reproduce quickly and they are supported by a large network of blood vessels. GBM is the […]